abstract：:Acinetobacter calcoaceticus LMD 79.41 produced significant amounts of pyrrolo-quinoline quinone (PQQ) in its culture medium when grown on quinic acid or shikimic acid. Studies with LMD 79.41 and PQQ- -mutants of this strain demonstrated that this organism contains an NAD(P)-independent quinate dehydrogenase (QDH) (EC ...

journal_title：Archives of microbiology

authors： van Kleef MA,Duine JA

更新日期：1988-05-01 00:00:00

abstract：:Two genes, aphE and orf, coding for putative Mr 29,000 and Mr 31,000, proteins respectively, were identified in the nucleotide sequence of a 2.8 kbp DNA segment cloned from Streptomyces griseus N2-3-11. The aphE gene expressed streptomycin (SM) resistance and a SM phosphorylating enzyme in S. lividans strains. The two...

journal_title：Archives of microbiology

authors： Heinzel P,Werbitzky O,Distler J,Piepersberg W

更新日期：1988-01-01 00:00:00

abstract：:Flow cytometry (FCM) in conjunction with immunocytochemical-labeling was used to analyze and screen a population of Escherichia coli clones containing a genomic library from the oil-degrading microorganism Acinetobacter calcoaceticus RAG-1 surface antigens. Reconstruction experiments using mixed populations indicated ...

journal_title：Archives of microbiology

authors： Minas W,Sahar E,Gutnick D

更新日期：1988-01-01 00:00:00

abstract：:Bacillus stearothermophilus strains PV 72 and ATCC 12980 carry a crystalline surface layer (S-layer) with hexagonal (p6) and oblique (p2) symmetry, respectively. Sites of insertions of new subunits into the regular lattice during cell growth have been determined by the indirect fluorescent antibody technique and the p...

journal_title：Archives of microbiology

authors： Gruber K,Sleytr UB

更新日期：1988-01-01 00:00:00

abstract：:Mannoproteins from cell walls of Saccharomyces cerevisiae synthesized at successive stages of the population growth cycle have been solubilized with Zymolyase and subsequently analyzed. The major change along the population cycle concerned a large size mannoprotein material; the size of the newly-synthesized molecules...

journal_title：Archives of microbiology

authors： Valentín E,Herrero E,Rico H,Miragall F,Sentandreu R

更新日期：1987-07-01 00:00:00

abstract：:The attachment of four species of bacteria to solid surfaces was investigated to determine whether the attachment of one species of bacterium could be influenced by the presence of other attaching or attached species. Three types of experiment were done: attachment of bacteria from suspensions containing two species (...

journal_title：Archives of microbiology

authors： McEldowney S,Fletcher M

更新日期：1987-06-01 00:00:00

abstract：:The regulatory elements involved in expression of the gene (fdhF) for the selenopolypeptide of formate dehydrogenase and of a gene (or transcriptional unit) (hyd) specifically responsible for the formation of the gas-evolving hydrogenase (hydrogenase 3) in Escherichia coli were investigated. Formate (or a product of i...

journal_title：Archives of microbiology

authors： Birkmann A,Zinoni F,Sawers G,Böck A

更新日期：1987-06-01 00:00:00

abstract：:Two ornithine carbamoyltransferases (OCT 1 and OCT 2) were isolated from Pseudomonas syringae pv. phaseolicola and purified by precipitation with ammonium sulfate, heat denaturation, chromatography on DEAE-Sephadex A-50 and Sephadex G-200. Molecular weights of both enzymes: 110,000; optimal activity: pH 8.5 to 9.5 (OC...

journal_title：Archives of microbiology

authors： Jahn O,Sauerstein J,Reuter G

更新日期：1987-03-01 00:00:00

abstract：:It has been shown previously that externally added glycine betaine is accumulated in Escherichia coli in response to the external osmotic strength. Here we have shown, by using nuclear magnetic resonance spectroscopy and radiochemical methods, that E. coli growing in a glucose-mineral medium of elevated osmotic streng...

journal_title：Archives of microbiology

authors： Larsen PI,Sydnes LK,Landfald B,Strøm AR

更新日期：1987-02-01 00:00:00

abstract：:An unidentified filamentous purple bacterium, probably belonging to a new genus or even a new family, is found in close association with the filamentous, mat-forming cyanobacterium Microcoleus chthonoplastes in a hypersaline pond at Guerrero Negro, Baja California Sur, Mexico, and in Solar Lake, Sinai, Egypt. This or...

journal_title：Archives of microbiology

authors： D'Amelio ED,Cohen Y,Des Marais DJ

更新日期：1987-01-01 00:00:00

abstract：:The pathway construction and allosteric regulation of phenylalanine and tyrosine biosynthesis was examined in Neisseria gonorrhoeae. A single 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase enzyme sensitive to feedback inhibition by L-phenylalanine was found. Chorismate mutase and prephenate dehydratase ap...

journal_title：Archives of microbiology

authors： Berry A,Jensen RA,Hendry AT

更新日期：1987-01-01 00:00:00

abstract：:The biosynthesis of the beta-glucosidase enzyme was studied in a transformed yeast obtained by cloning in Saccharomyces cerevisiae the structural gene coding for beta-glucosidase in Kluyveromyces fragilis. The enzyme biosynthesis was found to be non-adaptative, and repressed by glucose. These features are similar to t...

journal_title：Archives of microbiology

authors： Leclerc M,Chemardin P,Arnaud A,Ratomahenina R,Galzy P,Gerbaud C,Raynal A

更新日期：1986-11-01 00:00:00

abstract：:The fate of 35S label was studied during cystine degradation by mycelia of the dermatophytic fungus Microsporum gypseum. Excess free cystine in the medium was readily taken up and its sulfur moiety excreted as inorganic sulfate and sulfite. At intervals after 3-60 min of incubation with 35S cystine the products of cys...

journal_title：Archives of microbiology

authors： Kunert J,Trüper HG

更新日期：1986-07-01 00:00:00

abstract：:NAD kinase was purified 180-fold from Bacillus licheniformis to determine the role it plays in NADP turnover in this organism. The enzyme was found to have a pH optimum of 6.8 and an apparent Km for NAD of 2.7 mM. The ATP saturation curve was not hyperbolic; 5.5 mM ATP was required to reach half maximal activity. Both...

journal_title：Archives of microbiology

authors： Zerez CR,Moul DE,Andreoli AJ

更新日期：1986-05-01 00:00:00

abstract：:A LD-carboxypeptidase from Escherichia coli K 12 was isolated by Tris-EDTA treatment and purified to electrophoretic homogeneity by DEAE-cellulose chromatography. The enzyme has a molecular weight of approximately 12,000 as determined by sodium dodecyl sulfate-polyacrylamide electrophoresis and by Sephadex G-100 gel f...

journal_title：Archives of microbiology

authors： Metz R,Henning S,Hammes WP

更新日期：1986-03-01 00:00:00

abstract：:Saccharomyces cerevisiae mutants unable to grow at ethanol concentrations at which the wild type strain S288C does grow, have been isolated. Some of them show additional phenotypic alterations in colony size, temperature sensitivity and viability in ethanol, which cosegregate with the growth sensitivity in ethanol. 21...

journal_title：Archives of microbiology

authors： Aguilera A,Benítez T

更新日期：1986-01-01 00:00:00

abstract：:The mutagenic activity of ethyl acetate extracts of culture medium from Cunninghamella elegans incubated 72 h with various polycyclic aromatic hydrocarbons (PAHs) was evaluated in the Salmonella typhimurium reversion assay. All of the PAH extracts were assayed in tester strains TA98 and TA100 both with and without met...

journal_title：Archives of microbiology

authors： Cerniglia CE,White GL,Heflich RH

更新日期：1985-11-01 00:00:00

abstract：:Histoplasma capsulatum yeast cells have been studied by immunoelectron microscopy using rabbit polyclonal antisera and a biotin-avidin-peroxidase detection system. An antigenic surface layer has been visualized in the cell wall of immunostained organisms. This layer was not seen in samples prepared by standard electro...

journal_title：Archives of microbiology

authors： Edwards MR,Fritz KE

更新日期：1985-08-01 00:00:00

abstract：:The determination of enzymatic activities in cell-free extracts of Acidaminococcus fermentans and Peptostreptococcus asaccharolyticus led to a refined scheme for the pathway of glutamate fermentation via (R)-2-hydroxyglutarate to acetate and butyrate. From the ratio of these products the amount of ATP generated by sub...

journal_title：Archives of microbiology

authors： Wohlfarth G,Buckel W

更新日期：1985-07-01 00:00:00

abstract：:The site of integration of phage M mu d (Ap lac) in mutant M9s which leads to deficiency of formic dehydrogenase (benzylviologen-linked) activity was determined. It was shown that the phage had inserted into the gene for the seleno-polypeptide of the enzyme (80 kd) leading to the formation of a truncated peptide (60 k...

journal_title：Archives of microbiology

authors： Pecher A,Zinoni F,Böck A

更新日期：1985-05-01 00:00:00

abstract：:The main property of an Amt- (ammonium transport negative) mutant of Klebsiella pneumoniae is its inability to accumulate NH4+ intracellularly. When growing on nitrogen sources other than NH4+, the mutant constantly looses NH3 by diffusion. This loss results in poor growth. The NH3 excretion suggests the existence of ...

journal_title：Archives of microbiology

authors： Castorph H,Kleiner D

更新日期：1984-10-01 00:00:00

abstract：:Enzymes that are regulated by the ferredoxin/thioredoxin system in chloroplasts--fructose-1,6-bis-phosphatase (FBPase), sedoheptulose-1,7-bisphosphatase (SBPase), and phosphoribulokinase (PRK)--were partially purified from two different types of photosynthetic prokaryotes (cyanobacteria, purple sulfur bacteria) and te...

journal_title：Archives of microbiology

authors： Crawford NA,Sutton CW,Yee BC,Johnson TC,Carlson DC,Buchanan BB

更新日期：1984-10-01 00:00:00

abstract：:Effects of tunicamycin (TM) on the sexual agglutinability and zygote formation of Saccharomyces cerevisiae were studied using the two kinds of haploid strains, inducible and constitutive for sexual agglutinability. Induction of sexual agglutinability by opposite mating type sex pheromone of inducible strains was inhib...

journal_title：Archives of microbiology

authors： Hasegawa S,Yanagishima N

更新日期：1984-08-01 00:00:00

abstract：:Bacillus cereus ATCC 14579 transformed naphthalene predominately to 1-naphthol. Experiments with [14C]naphthalene showed that over a 24 h period, B. cereus oxidized 5.2% of the added naphthalene. 1-Naphthol accounted for approximately 80% of the total metabolites. B. cereus incubated with naphthalene under the presenc...

journal_title：Archives of microbiology

authors： Cerniglia CE,Freeman JP,Evans FE

更新日期：1984-08-01 00:00:00

abstract：:Rhodopseudomonas capsulata produces both an intermediate (I) and a large (L) form of ribulose-1,5-bisphosphate carboxylase/oxygenase. Both forms are derepressed under CO2-limiting conditions. The L-form of the enzyme is completely repressed when the culture is grown either photoautotrophically or photoheterotrophicall...

journal_title：Archives of microbiology

authors： Shively JM,Davidson E,Marrs BL

更新日期：1984-07-01 00:00:00

abstract：:Calcium was found to stimulate stalk development in Caulobacter crescentus and to relieve the inhibition of development long known to be caused by phosphate. This suggested that phosphate inhibition could be attributed to its interaction with Ca2+, thereby depriving the cells of a factor that promoted development. Cal...

journal_title：Archives of microbiology

authors： Poindexter JS

更新日期：1984-06-01 00:00:00

abstract：:Representative strains of Gardnerella vaginalis were degraded using both an alkaline and an acid methanolysis and the fatty acid methyl esters released examined by thin-layer and gas chromatography. The profiles obtained were both qualitatively and quantitatively similar and were comprised of straight chain saturated ...

journal_title：Archives of microbiology

authors： O'Donnell AG,Minnikin DE,Goodfellow M,Piot P

更新日期：1984-05-01 00:00:00

abstract：:Coenzyme F420 is a 8-hydroxy-5-deazaflavin present in methanogenic bacteria. We have investigated whether the pyrimidine ring of the deazaflavin originates from guanine as in flavin biosynthesis, in which the pyrimidine ring of guanine is conserved. For this purpose the incorporation of [2-14C]guanine and of [8-14C]gu...

journal_title：Archives of microbiology

authors： Jaenchen R,Schönheit P,Thauer RK

更新日期：1984-04-01 00:00:00

abstract：:A total of 13 killer toxin producing strains belonging to the genera Saccharomyces, Candida and Pichia were tested against each other and against a sensitive yeast strain. Based on the activity of the toxins 4 different toxins of Saccharomyces cerevisiae, 2 different toxins of Pichia and one toxin of Candida were reco...

journal_title：Archives of microbiology

authors： Pfeiffer P,Radler F

更新日期：1984-04-01 00:00:00

abstract：:The disaccharide sophorose induces Trichoderma to increase a soluble intracellular b-glucosidase that hydrolyses cellobiose, sophorose, and p-nitrophenyl-b-D-glucopyranoside. Simultaneously, it depresses the activity of a similar insoluble enzyme that is associated with the mycelium. Gel electrophoresis indicates that...

journal_title：Archives of microbiology

authors： Loewenberg JR

更新日期：1984-01-01 00:00:00

abstract：:Two different acidic polysaccharides (I and II) were detected in the external cell envelope layers (slime and capsule) of Rhodopseudomonas capsulata Sp11. Polysaccharide I contains rhamnose, fucose, glucosamine and an unknown acidic sugar, it represents the slime material of the strain. Polysaccharide II contains rham...

journal_title：Archives of microbiology

authors： Omar AS,Weckesser J,Mayer H

更新日期：1983-12-01 00:00:00

abstract：:Pyrophosphate (PPi) content of Escherichia coli is increased manyfold when the growth of the cells is limited by inhibition of the synthesis of nucleotides. The accumulated PPi is immediately degraded when inhibition is released and growth allowed to resume. Other tested nutritional deficiencies (starvation of carbon ...

journal_title：Archives of microbiology

authors： Kukko E,Saarento H

更新日期：1983-11-01 00:00:00

abstract：:Azotobacter vinelandii was grown in oxygen-controlled continuous cultures under conditions of dinitrogen fixation. Different oxygen concentrations were adjusted with air. Cell-free extracts were employed to study the oxygen dependency of the intracellular distribution and activity of the following enzymes: nitrogenase...

journal_title：Archives of microbiology

authors： Röckel D,Hernando JJ,Vakalopoulou E,Post E,Oelze J

更新日期：1983-10-01 00:00:00

abstract：:Enoate reductase present in Clostridium kluyveri and Clostridium spec. La 1 could be detected in three strains of C. tyrobutyricum and ten clostridia belonging to the groups of proteolytic and saccharolytic or proteolytic species, respectively. In C. pasteurianum, C. butyricum and C. propionicum enoate reductase could...

journal_title：Archives of microbiology

authors： Giesel H,Simon H

更新日期：1983-08-01 00:00:00

abstract：:Peptidoglycan of Corynebacterium species Co 112 (DSM 20606) exhibits an unknown amino acid. The amino acid was isolated from cell wall hydrolysates and identified as threo-beta-hydroxyornithine. This amino acid is found in the interpeptide bridge of the peptidoglycan of Corynebacterium sp. Co 112. The primary structur...

journal_title：Archives of microbiology

authors： Schleifer KH,Hayn I,Seidl HP,Firl J

更新日期：1983-06-01 00:00:00

abstract：:Inactivation of peroxisomal enzymes in the yeast Hansenula polymorpha was studied following transfer of cells into cultivation media in which their activity was no longer required for growth. After transfer of methanol-grown cells into media containing glucose - a substrate that fully represses alcohol oxidase synthes...

journal_title：Archives of microbiology

authors： Veenhuis M,Douma A,Harder W,Osumi M

更新日期：1983-06-01 00:00:00

abstract：:Plasmid pJP4 encoding the ability to degrade the herbicide 2,4-dichlorophenoxyacetic acid (Tfd+) was transferred by conjugation from Escherichia coli JMP397 to various lithoautotrophic strains of Alcaligenes eutrophus and to the autotrophic bacterium Pseudomonas oxalaticus. The herbicide-degrading function of the plas...

journal_title：Archives of microbiology

authors： Friedrich B,Meyer M,Schlegel HG

更新日期：1983-02-01 00:00:00

abstract：:The induction by D,L-nicotine of the enantiozymes 6-hydroxy-L-nicotine oxidase and 6-hydroxy-D-nicotine oxidase in Arthrobacter oxidans was differently affected by the inhibitors of Escherichia coli gyrase, novobiocin and nalidixic acid. These compounds inhibited 6-hydroxy-L-nicotine oxidase induction slightly, but le...

journal_title：Archives of microbiology

authors： Brandsch R,Decker K

更新日期：1982-12-03 00:00:00

abstract：:Hopane glycolipid, isolated from Bacillus acidocaldarius, was studied in its ability to support growth from Mycoplasma mycoides var. capri as substitute for sterols. All attempts to culture Mycoplasma on hopane glycolipid failed. When added together with cholesterol, the effect of HGL was a reduction in growth rate. T...

journal_title：Archives of microbiology

authors： Kannenberg E,Poralla K

更新日期：1982-12-01 00:00:00

abstract：:1. With fumarate as the terminal electron acceptor and either H2 or formate as donor, Vibrio succinogenes could grow anaerobically in a mineral medium using fumarate as the sole carbon source. Both the growth rate and the cell yield were increased when glutamate was also present in the medium. 2. Glutamate was incorpo...

journal_title：Archives of microbiology

authors： Bronder M,Mell H,Stupperich E,Kröger A

更新日期：1982-05-01 00:00:00